切片機是目前國內研制生產(chǎn)的產(chǎn)品,它廣泛地應用于科研、教學(xué)、化工、醫療衛生等單位,專(zhuān)切新鮮的或經(jīng)過(guò)固定的動(dòng)、植物標本,特別是新鮮的腦組織。
Slicer is a product developed and produced in our country. It is widely used in scientific research, teaching, chemical industry, medical and health units. It specially cuts fresh or fixed animal and plant specimens, especially fresh brain tissue.
切片機切片時(shí)組織標本不需冰凍或包埋,為此,樣品片避免了冰晶破壞,還能保持樣品活性和細胞良好形態(tài),給“免疫細胞化學(xué)研究 ”以及“脊髓和腦薄片的神經(jīng)生物學(xué)研究”提供了良好條件,是當代電鏡、解剖、組胚、生理、醫院、化工等實(shí)驗系統zui理想的快速制樣理想切片儀器。切片機經(jīng)過(guò)科學(xué)院上海生理研究所、復旦大學(xué)、第二軍醫大學(xué)、北京大學(xué)、北京協(xié)和醫院、廣州中山大學(xué)等單位使用證明,切片機切固定的腦和脊髓等組織,zui薄可切到10微米,新鮮的腦、心、腎等組織zui薄可切到30微米,且切片完整,片面光滑,染色均勻,其主要技術(shù)指標達到國外同類(lèi)產(chǎn)品水平。
In order to avoid the destruction of ice crystal and keep the activity of the sample and the good shape of the cell, we need not freeze or embed the tissue specimen when slicing by the microtome ”And "neurobiological study of spinal cord and brain slice" provides good conditions, which is an ideal instrument for rapid sample preparation of Zui experimental system of electron microscopy, anatomy, embryo formation, physiology, hospital, chemical industry, etc. The slicer has been used by Shanghai Institute of physiology, Fudan University, the Second Military Medical University, Peking University, Peking Union Medical College, Guangzhou Zhongshan University and other units. It has been proved that the slicer can cut the fixed brain and spinal cord and other tissues. The thin Zui can be cut to 10 microns. The thin Zui of fresh brain, heart, kidney and other tissues can be cut to 30 microns. The slicer is complete, one-sided and smooth, with uniform staining The main technical indicators have reached the level of similar products abroad.
通常來(lái)講,切片機的操作闡明包含開(kāi)機和切片這兩部分。開(kāi)啟切片機主機電源,翻開(kāi)開(kāi)關(guān),運用箱體溫度設置按鈕設置切片時(shí)所需的溫度,這時(shí)溫度顯現窗口會(huì )同步顯現設置的溫度。設置好后,過(guò)5秒后,會(huì )顯現實(shí)踐溫度。同時(shí),咱們也能夠隨時(shí)查看設置的溫度巨細。*次開(kāi)機時(shí),切片機應經(jīng)過(guò)基準時(shí)刻設置按鈕設置一個(gè)基準時(shí)刻,經(jīng)過(guò)除霜時(shí)刻設置按鈕來(lái)設置除霜時(shí)刻。假如需求手動(dòng)除霜,則應先按下手動(dòng)除霜按鈕,聽(tīng)到峰鳴音后,按下疾速制冷按鈕可進(jìn)行冷凍臺的除霜,按下箱體溫度設置按鈕則是箱體除霜。如要關(guān)閉,只要按此次序再按一次就能夠了。
Generally speaking, the operation of the slicer includes two parts: startup and slicing. Turn on the main power of the slicer, turn on the switch, and use the box temperature setting button to set the required temperature for slicing. At this time, the temperature display window will synchronously display the set temperature. After setting, after 5 seconds, the practice temperature will appear. At the same time, we can check the temperature at any time. *During the secondary startup, the slicer shall set a reference time through the setting button of the reference time, and set the defrosting time through the setting button of the defrosting time. If manual defrosting is required, press the manual defrosting button first. After hearing the peak sound, press the fast cooling button to defrost the freezer. Press the box temperature setting button to defrost the box. To close, just press again in this order.
切片機能夠運用厚度調理旋鈕來(lái)調理切片厚度,裝置刀片時(shí)應調整好切片角度。切片前,先將樣品進(jìn)行冷卻,要冷卻透徹,然后放到切片機上,樣品要接近刀口,并調整好平面。以后按下按鈕,進(jìn)行切片,切出的樣品要平坦的進(jìn)入防卷板與刀片之間的空地,zui終取出,進(jìn)行染色觀(guān)察。想要避免切片機的參數被更改,能夠將鍵盤(pán)確定,經(jīng)過(guò)控制面板上的鍵盤(pán)確定按鈕。按下按鈕5秒鐘左右,當時(shí)刻窗口中的兩個(gè)點(diǎn)中止閃耀時(shí),就表明鍵盤(pán)現已被確定了,想要解鎖辦法同上。切片機再次開(kāi)機前,應查看切片機內是不是有積水,如有應處理潔凈后再開(kāi)機,假如需求長(cháng)時(shí)刻運用,那么可將其夜間的溫度調零下十度以下,這么有利于維護切片機。
The slicer can use the thickness adjusting knob to adjust the slice thickness, and the slice angle should be adjusted when the blade is installed. Before slicing, first cool the sample thoroughly, and then put it on the slicer. The sample should be close to the knife edge, and the plane should be adjusted. After that, press the button to slice. The cut sample should enter the open space between the anti roll plate and the blade, and Zui finally takes it out for dyeing observation. To avoid the parameters of the slicer being changed, the keyboard can be determined through the keyboard determination button on the control panel. Press the button for about 5 seconds, when two points in the window stop flashing, it means that the keyboard has been determined, and the way to unlock is the same as above. Before the slicer is turned on again, check whether there is water in the slicer. If there is water in the slicer, clean it and then turn it on again. If it needs to be used for a long time, then adjust its temperature at night to below - 10 ℃, which is conducive to maintaining the slicer.
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